Molecular identification of Tuber magnatum ectomycorrhizae in the field
نویسندگان
چکیده
منابع مشابه
Cloning and characterization of PKC-homologous genes in the truffle species Tuber borchii and Tuber magnatum.
The protein kinases C (PKCs) define a growing family of ubiquitous signal transducting serine/threonine kinases that control ion conductance channels, release of hormones and cell growth and proliferation. Degenerated oligonucleotides were used as primers for polymerase chain reactions to amplify PKC-related sequences from the white truffle species Tuber magnatum and Tuber borchii. The deduced ...
متن کاملReevaluation of the life cycle of Tuber magnatum.
Tuber spp. are ectomycorrhizal ascomycetes that produce ascocarps known as truffles. Basic aspects of Tuber biology have yet to be fully elucidated. In particular, there are conflicting hypotheses concerning the mating system and the ploidy level of the mycorrhizal and truffle hyphae. We used polymorphic microsatellites to compare the allelic configurations of asci with those from the network o...
متن کاملGenetic and phylogeographic structures of the symbiotic fungus Tuber magnatum.
The quality and market price of truffles vary with the species and, traditionally, the place of origin. The premium species Tuber magnatum produces white truffles and has a patchy distribution restricted to Italy and some Balkan areas. We used polymorphic microsatellites to evaluate 316 specimens grouped into 26 populations sampled across the species' geographic range to determine if natural po...
متن کاملDNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.
The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OP...
متن کاملSpatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Tuber magnatum produces the world's most expensive truffle. This fungus produces very rare ectomycorrhizas which are difficult or even impossible to detect in the field. A "real-time" PCR assay was recently developed to quantify and to track T. magnatum mycelium in soil. Here, this technique was used to investigate the spatial distribution of T. magnatum extra-radical mycelium in soil productiv...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2006
ISSN: 0944-5013
DOI: 10.1016/j.micres.2005.06.003